Description

This book of “cancer drug resistance” discusses the latest advancements and technologies used in cancer drug resistance research. ”Cancer Drug Resistance” contains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging therapeutic drugs; studies to delineate the role of efflux transporters; expression of drug transporters; resistance to targeted therapies in breast cancer; the role of microRNAs in current pancreatic cancer treatment; and cancer exosomes as mediators of drug resistance or clinical and molecular methods in drug development and the use of bioinformatics in the management of cancer drug resistance data. cancer drug resistance is a major obstacle to achieving a cancer cure. Resistance to chemotherapeutic and molecularly targeted therapies can be intrinsic, dependent on the genetic makeup of the patient and involving molecular changes that have led to the development of the cancer. Drug therapies also provide strong evolutionary pressures on cancer cells, pressures which ultimately result in genetic, epigenetic and other molecular changes that promote adaptation of cancer cells to the therapeutic challenge, or selection for initially rare, intrinsically resistant cancer cells in a heterogeneous tumour cell population. . Written in the highly successful Methods in Molecular Biology series format, chapters include overviews of the main issues in cancer drug resistance and the respective mechanisms, as well as introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.